14-3-3 sigma/SFN antibody (C-Term)

Catalog No. ABIN5541548

This Rabbit Polyclonal antibody specifically detects 14-3-3 sigma/SFN in WB, IF, FACS, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for 14-3-3 sigma/SFN antibody (C-Term) (ABIN5541548)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 sigma/SFN antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-14-3-3 sigma/SFN Antibody

Binding Specificity: C-Term

Specificity: This antibody recognizes Human SFN (C-term). Other species not tested.

Purification: Protein A column, followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide between 222-248 amino acids from the C-terminal region of Human SFN.

Isotype: Ig Fraction

Application Details

Application Notes: Western blotting: 1/1000. Immunohistochemistry on Paraffin Sections: 1/10-1/50. Flow Cytometry: Immunofluorescence:

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS Preservatives: 0.09 % (W/V) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: 14-3-3 protein sigma,sfn

Background: Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axolly transported to the nerve termils. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of sigl transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in sigling pathways mediated by serine/threonine protein kises. Evidence indicates 14-3-3 is required for Raf 1 kise activity and phosphorylation among many other functions.

UniProt: P31947

Pathways: p53 Signaling, Myometrial Relaxation and Contraction