GOT1 antibody (HRP)

Catalog No. ABIN5541603

The Sheep Polyclonal anti-GOT1 antibody is suitable to detect GOT1 in samples from Pig. It has been validated for WB and EIA.

Quick Overview for GOT1 antibody (HRP) (ABIN5541603)

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Reactivity: Pig

Host: Sheep

Clonality: Polyclonal

Conjugate: This GOT1 antibody is conjugated to HRP

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-GOT1 Antibody (HRP)

Specificity: This antibody detects porcine GOT1. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase and anti-sheep serum as well as purified and partially purified aspartate transaminase / GOT1 [porcine heart]. Species: Porcine (Pig).

Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer

Immunogen: Aspartate aminotransferase / GOT1 from porcine heart

Isotype: IgG

Application Details

Application Notes: Western blot : 1/1,000-1/5,000. ELISA : 1/10,000-1/40,000. This antibody has been assayed against 1.0 μg of aspartate transaminase [porcine heart] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GOT1

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Alternative Name: got1

Background: Aspartate aminotransferase (Glutamate oxaloacetate transaminase / GOT1) is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80 % of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene. Serum GOT (with SGPT) levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.

UniProt: P00503

Pathways: Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process