GRIN2B antibody (C-Term)

Catalog No. ABIN5541843

The Rabbit Polyclonal anti-GRIN2B antibody is suitable to detect GRIN2B in samples from Human, Rat and Mouse. It has been validated for WB, IP and IHC (fro).

Quick Overview for GRIN2B antibody (C-Term) (ABIN5541843)

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIN2B antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GRIN2B Antibody

Binding Specificity: C-Term

Specificity: Specific for the ~180k NR2B subunit of the NMDA receptor. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody. No reactivity towards the NR2A and NR2C subunits.

Purification: Affinity purification using a column to which the fusion protein immunogen was coupled

Immunogen: Fusion protein from the C-terminus of the NR2B subunit of rat NMDA receptor

Isotype: Ig Fraction

Application Details

Application Notes: Western blot: 1/1000. Immunohistochemistry on frozen sections: 1/1000 - 1/2000. Immunoprecipitaion: 6 μL per 200 μL lysate.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg BSA per ml and 50 % glycerol

Storage: -20 °C

Storage Comment: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GRIN2B

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Alternative Name: nmdar2b

Background: The ion channels activated by glutamat e that are sensitive to N-methyl-D- aspartate (NMDA) are designated NMDA receptors (N MDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been im plicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002 Wenthold et al., 2003 Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989 Alvestad et al., 2003 Snell et al., 1996). The rat NMDAR1 (NR1) was the first subunit of the NM DAR to be cloned and it can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological char acteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Overexpression of the NR2B-subunit of the NMDA receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expres sion (Clayton et al., 2002a Clayton et al., 2002b). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999).

UniProt: Q00960

Pathways: Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity