NANOS3 antibody (C-Term)

Catalog No. ABIN500324

Product Details anti-NANOS3 Antibody

Target: NANOS3 (Nanos Homolog 3 (NANOS3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NANOS3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects Nanos3 at C-term. It will not cross-react with either Nanos 1 or Nanos2.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: Nanos3 antibody was raised against a 16 amino acid peptide near the carboxy terminus of the human Nanos3.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for NANOS3

Target: NANOS3 (Nanos Homolog 3 (NANOS3))

Alternative Name: NANOS3 (NANOS3 Products)

Synonyms: NANOS1L antibody, NOS3 antibody, nos3 antibody, cb725 antibody, nanos antibody, nanos1 antibody, nos1 antibody, nanos C2HC-type zinc finger 3 antibody, nanos homolog 3 (Drosophila) antibody, nanos homolog 3 antibody, NANOS3 antibody, Nanos3 antibody, nanos3 antibody

Background: Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects.Synonyms: MGC120114, NANOS1L, NOS3, Nanos homolog 3

Gene ID: 342977

NCBI Accession: NP_001092092

UniProt: Q495E5

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling