Recombinant Cyclin D1 antibody (C-Term)

Catalog No. ABIN5557293

This Rabbit Monoclonal antibody specifically detects Cyclin D1 in WB, FACS, IF, ICC, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Recombinant Cyclin D1 antibody (C-Term) (ABIN5557293)

Target: Cyclin D1 (CCND1)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Cyclin D1 Antibody

Binding Specificity: C-Term

Purpose: Cyclin D1 Recombinant Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant human Cyclin D1 protein, around C-terminal 150aa.

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(1:50-200), Flow-Cyt(1:50-100), ICC/IF(1:50-100)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: Cyclin D1

Background:

Synonyms: Cyclin D1 phospho T286, p-Cyclin D1 phospho T286, AI327039, B cell ccl/lymphoma 1, B cell leukemia 1, B-cell CLL/lymphoma 1, B-cell leukemia 1, B-cell lymphoma 1 protein, BCL-1, BCL1, BCL1 oncogene, CCND 1, CCND1, CCND1 protein, CCND1/FSTL3 fusion gene, included, CCND1/IGHG1 fusion gene, CCND1/IGHG1 fusion gene, included, CCND1/IGLC1 fusion gene, included, CCND1/PTH fusion gene, included, cD1, Cyclin D1, Cyl-1, D11S287E, G1/S specic cyclin D1, MGC137744, Parathyroid adenomatosis 1, PRAD1, PRAD1 oncogene, U21B31, CCND1_HUMAN.

Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008].

Gene ID: 595

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling