Recombinant Cyclin E1 antibody

Catalog No. ABIN5557295

The Rabbit Monoclonal anti-Cyclin E1 antibody (ABIN5557295) specifically detects Cyclin E1 in WB, IF, IHC (p), ICC and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for Recombinant Cyclin E1 antibody (ABIN5557295)

Target: Cyclin E1 (CCNE1)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cyclin E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Cyclin E1 Antibody

Purpose: Cyclin E1 Recombinant Antibody

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: Synthetic peptide derive from human cyclin e1

Isotype: IgG

Application Details

Application Notes: WB(1:1000-5000), IHC-P(1:200-500), IHC-F(1:200-500), IF(1:200-500), ICC/IF(1:100-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: Cyclin E1

Background:

Synonyms: Cyclin E1 phospho T77, p-Cyclin E1 phospho T77, Cyclin E1 Thr77, CCNE 1, CCNE,CCNE1,Cyclin E1 antibody Cyclin Es,Cyclin Et,CyclinE, G1/S specic cyclin E.

Background: Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

Gene ID: 898

UniProt: P24864

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases