TET1 antibody (AA 1682-1914)

Catalog No. ABIN5563987

Cited in 2+ publications. The Rat Monoclonal anti-TET1 antibody (Clone 5D6) (ABIN5563987) specifically detects TET1 in WB, IF, IP, ChIP-seq and ICC. The antibody is reactive with Human and Mouse samples.

Quick Overview for TET1 antibody (AA 1682-1914) (ABIN5563987)

Target: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TET1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq), Immunocytochemistry (ICC)

Clone: 5D6

Product Details anti-TET1 Antibody

Binding Specificity: AA 1682-1914

Purpose: Tet1 antibody (mAb) (Clone 5D6)

Specificity: Mouse

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a recombinant protein corresponding amino acids 1682-1914 of mouse Tet1.

Isotype: IgG2a

Application Details

Application Notes: ChIP-Seq: 4 µL per ChIP This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91171.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-TET1 antibody (ABIN5563987)

Thaler, Maurizi, Roschger, Sturmlechner, Khani, Spitzer, Rumpler, Zwerina, Karlic, Dudakovic, Klaushofer, Teti, Rucci, Varga, van Wijnen: "Anabolic and Antiresorptive Modulation of Bone Homeostasis by the Epigenetic Modulator Sulforaphane, a Naturally Occurring Isothiocyanate." in: The Journal of biological chemistry, Vol. 291, Issue 13, pp. 6754-71, (2016) (PubMed).

Bauer, Göbel, Nagaraj, Colantuoni, Wang, Müller, Kremmer, Rottach, Leonhardt: "Phosphorylation of TET proteins is regulated via O-GlcNAcylation by the O-linked N-acetylglucosamine transferase (OGT)." in: The Journal of biological chemistry, Vol. 290, Issue 8, pp. 4801-12, (2015) (PubMed).

Target Details for TET1

Target: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Alternative Name: TET1

Background: Tet1 (Ten-Eleven Translocation-1, methylcytosine dioxygenase) is a protein that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) which can lead to cytosine demethylation by either further oxidation via Tet proteins to 5-formylcytosine (5fC) and 5-carboxycytosine (5caC) or deamination into 5-hydroxymethyluracil (5hmU) and then subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. Tet1 preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells, it plays a role in embryonic stem cells maintenance and inner cell mass cell specification.

Molecular Weight: 215 kDa

NCBI Accession: NP_001240786

Pathways: Stem Cell Maintenance, Warburg Effect