APOC3 antibody

Catalog No. ABIN5596680

Product Details anti-APOC3 Antibody

Target: APOC3 (Apolipoprotein C-III (APOC3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Cross-Reactivity (Details): Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein C-III and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

Purification: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.

Immunogen:

Immunogen: produced from apoLipoprotein Type C-III derived from human plasma

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Anti-Apolipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, and for western blotting for highly sensitive qualitative analysis. When performing immunoblotting assays - do not block with blotto. Blotto formulations tend to contain apoLipoprotein Type C-III and will neutralize antibody activity.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for anti-APOC3 antibody (ABIN5596680)

Jansen, Cirak, van Scherpenzeel, Timal, Reunert, Rust, Pérez, Vicogne, Krawitz, Wada, Ashikov, Pérez-Cerdá, Medrano, Arnoldy, Hoischen, Huijben, Steenbergen, Quelhas, Diogo, Rymen, Jaeken, Guffon et al.: "CCDC115 Deficiency Causes a Disorder of Golgi Homeostasis with Abnormal Protein Glycosylation. ..." in: American journal of human genetics, Vol. 98, Issue 2, pp. 310-21, (2016) (PubMed).

Takinami, Yoshimatsu, Uchiumi, Toyosaki-Maeda, Morita, Ishihara, Yamane, Fukuda, Okamoto, Numata, Fukui: "Identification of potential prognostic markers for knee osteoarthritis by serum proteomic analysis." in: Biomarker insights, Vol. 8, pp. 85-95, (2013) (PubMed).

Target Details for APOC3

Target: APOC3 (Apolipoprotein C-III (APOC3))

Alternative Name: APOLIPOPROTEINC-III (APOC3 Products)

Synonyms: APOC3 antibody, APOCIII antibody, HALP2 antibody, Apoc antibody, ApoC-III antibody, apo-CIII antibody, Apo-CIII antibody, apolipoprotein C3 antibody, apolipoprotein C-III antibody, APOC3 antibody, Apoc3 antibody

Background:

Synonyms: ApoCIII antibody, Apolipoprotein C III antibody

Background: Anti Apolipoprotein C-III antibody recognizes the gene product of APOC. Apolipoprotein C-III is a protein component of very low density lipoprotein (VLDL). APOC3 inhibits lipoprotein lipase and hepatic lipase, it is thought to inhibit hepatic uptake of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia. This antibody is suitable for cardiovascular research.

Gene Name: APOC3

Gene ID: 345

UniProt: P02656

Pathways: Carbohydrate Homeostasis, Lipid Metabolism