PCK1 antibody (HRP)
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- Target See all PCK1 Antibodies
- PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
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Reactivity
- Zea mays
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PCK1 antibody is conjugated to HRP
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Cross-Reactivity (Details)
- Cross reactivity against Phospho-enol-Pyruvate Carboxylase from other tissues and species may occur but have not been specifically determined.
- Purification
- Phospho Enol Pyruvate Carboxylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Phospho-enol-Pyruvate Carboxylase [Maize].
- Immunogen
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Immunogen: Phospho-enol-pyruvate Carboxylase [Maize Leaves]
Immunogen Type: Native Protein
- Isotype
- IgG
- Top Product
- Discover our top product PCK1 Primary Antibody
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- Application Notes
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Phosphoenolpyruvate Carboxylase has been assayed against 1.0 μg of Phospho-enol-Pyruvate Carboxylase [Maize] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:5,000 - 1:20,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Preservative
- Gentamicin sulfate
- Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
- Alternative Name
- Phospho Enol Pyruvate Carboxylase (PCK1 Products)
- Synonyms
- PEPCK-C antibody, PEPCK1 antibody, PEPCKC antibody, PEPCK antibody, PEPCK-M antibody, PEPCK2 antibody, 1810010O14Rik antibody, 9130022B02Rik antibody, cb856 antibody, cb924 antibody, fj93h11 antibody, zgc:63869 antibody, wu:fc51c05 antibody, wu:fj93h11 antibody, pepck-c antibody, pepck1 antibody, pepckc antibody, PCK1 antibody, PPCK1 antibody, AI265463 antibody, Pck-1 antibody, GTP antibody, PCK antibody, Pepck antibody, RATPEPCK antibody, Ppc1C antibody, PHOSPHOENOLPYRUVATE CARBOXYKINASE antibody, T28I19.150 antibody, T28I19_150 antibody, phosphoenolpyruvate carboxykinase 1 antibody, 143299_at antibody, CG10924 antibody, CG17725 antibody, Dmel\\CG17725 antibody, Dromel_CG17725_FBtr0086701_pepck_mORF antibody, dPEPCK antibody, pepck antibody, PEPC antibody, PEPCase antibody, ppc antibody, phosphoenolpyruvate carboxykinase 1 antibody, phosphoenolpyruvate carboxykinase 2, mitochondrial antibody, phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody, phosphoenolpyruvate carboxykinase 1 (soluble) antibody, phosphoenolpyruvate carboxykinase 1 S homeolog antibody, phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody, phosphoenolpyruvate carboxykinase 1, cytosolic antibody, phosphoenolpyruvate carboxylase 7 antibody, Phosphoenolpyruvate carboxykinase antibody, phosphoenolpyruvate carboxylase antibody, PCK1 antibody, PCK2 antibody, Pck2 antibody, pck1 antibody, pck1.S antibody, LOC100634531 antibody, Pck1 antibody, pep7 antibody, Pepck antibody, LOC107777405 antibody
- Background
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Synonyms: PEPC 1 antibody, PEPCase 1 antibody, Phosphoenolpyruvate carboxylase 1 antibody
Background: Phospho Enol Pyruvate Carboxylase is an enzyme in the family of carboxy-lyases that catalyzes the addition of bicarbonate to phosphoenolpyruvate (PEP) to form a four-carbon compound oxaloacetate. This reaction is used for carbon fixation in CAM and C4 plants where it plays a key role in photosynthesis. The enzyme is also found in some bacteria, but not in animals or fungi.
Gene Name: PEPC
- Gene ID
- 542372
- UniProt
- B8XPZ2
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
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