Collagen IV antibody (Biotin)

Catalog No. ABIN5596836

Supplier Product No.: 600-406-106

There are 2+ publications for this product available. The Rabbit Polyclonal anti-Collagen IV antibody is suitable to detect Collagen IV in samples from Human and Cow. It has been validated for WB, ELISA, IHC and IP.

Quick Overview for Collagen IV antibody (Biotin) (ABIN5596836)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-Collagen IV Antibody (Biotin)

Supplier Product No.: 600-406-106

Supplier: Rockland

Purpose: Collagen Type IV Antibody Biotin Conjugated

Cross-Reactivity (Details): Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Characteristics: Synonyms: rabbit anti-Collagen Type IV antibody biotin conjugation, biotin conjugated rabbit anti-Collagen Type IV antibody, Arresten antibody, Canstatin antibody, Collagen Of Basement Membrane Alpha 1 Chain antibody, Collagen alpha-1 (IV) chain, COL4A1

Purification: Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.

Immunogen:

Immunogen: Anti-Collagen Type IV was produced by repeated immunizations with Collagen Type IV from human and bovine placenta.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Collagen Type IV is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:2,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Collagen IV antibody (Biotin) (ABIN5596836)

Cohen, Hadas, Stefania, Pagoto, Ben-Dor, Kohen, Longo, Elbaz, Dekel, Gershon, Aime, Neeman: "Magnetic Resonance Imaging Reveals Distinct Roles for Tissue Transglutaminase and Factor XIII in Maternal Angiogenesis During Early Mouse Pregnancy." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 39, Issue 8, pp. 1602-1613, (2020) (PubMed).

Aguilar, Gardiner: "DNA Methylation Dynamics Regulate the Formation of a Regenerative Wound Epithelium during Axolotl Limb Regeneration." in: PLoS ONE, Vol. 10, Issue 8, pp. e0134791, (2016) (PubMed).

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen Type IV

Background: Background: Anti-Collagen Type IV detects collagen. Type-IV collagen is a type of collagen found primarily in the basal lamina. It is the major structural element of basal membranes. Anti-Collagen Type IV antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.

Gene ID: 1282

NCBI Accession: NP_001290039

UniProt: P02462