GPD1 antibody (HRP)

Catalog No. ABIN5596858

Product Details anti-GPD1 Antibody (HRP)

Target: GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This GPD1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Glycerol-3-Phosphate-Dehydrogenase from other sources is unknown.

Purification: Glycerol-3-Phosphate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle].

Immunogen:

Immunogen: Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Glycerol-3-Phosphate Dehydrogenase has been assayed against 1.0 μg of Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GPD1

Target: GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))

Alternative Name: Glycerol-3-Phosphate Dehydrogenase (GPD1 Products)

Synonyms: GPDH antibody, Gpd3 antibody, GPD-C antibody, GPDH-C antibody, HTGTI antibody, CG9042 antibody, DROGPDHA antibody, DmG3PDH antibody, Dmel\\CG9042 antibody, G3PDH antibody, G3pdh antibody, GAPDH antibody, GPD antibody, GPDA antibody, GPDH-1 antibody, Gdh antibody, Gpd antibody, alpha-GPD antibody, alpha-GPDH antibody, alpha-GPDH-1 antibody, alpha-Gpdh antibody, alphaGPDH antibody, alphaGpd antibody, alphaGpdh antibody, alphaGpdh-1 antibody, gpdh antibody, gpdh-1 antibody, sn-Gpdh antibody, AI747587 antibody, Gdc-1 antibody, Gdc1 antibody, mKIAA4010 antibody, gpd1 antibody, wu:fc30a07 antibody, zgc:63859 antibody, MGC79676 antibody, GPD1 antibody, LG3P antibody, zgc:112197 antibody, Gpd1 antibody, gpd1h antibody, gpd1l antibody, wu:fc58b05 antibody, zgc:66051 antibody, zgc:85742 antibody, glycerol-3-phosphate dehydrogenase 1 antibody, Glycerol-3-phosphate dehydrogenase [NAD(+)] antibody, glycerol-3-phosphate dehydrogenase antibody, Glycerol 3 phosphate dehydrogenase antibody, glycerol-3-phosphate dehydrogenase 1 (soluble) antibody, glycerol-3-phosphate dehydrogenase 1 L homeolog antibody, glycerol-3-phosphate dehydrogenase 1c antibody, glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic antibody, glycerol-3-phosphate dehydrogenase 1a antibody, glycerol-3-phosphate dehydrogenase 1b antibody, Gpd1 antibody, gpdh-1 antibody, GPD1 antibody, Tb11.02.5280 antibody, Gpdh antibody, gpd1.L antibody, gpd1c antibody, gpd1 antibody, LOC101071719 antibody, gpd1a antibody, gpd1b antibody

Background:

Synonyms: EC 1.1.1.8 antibody, FLJ26652 antibody, G3PD antibody, Gdc-1 antibody, Glycerphosphate dehydrogenase antibody, GPD-C antibody, Gpd1 protein antibody

Background: Glycerol-3-phosphate dehydrogenase serves as a major link between carbohydrate metabolism and lipid metabolism. Through the reduction of dihydroxyacetone phosphate into glycerol 3-phosphate, GPDH allows the prompt dephosphorylation of glycerol 3-phosphate into glycerol. It is also a major contributor of electrons to the electron transport chain in the mitochondria. GPDH is responsible for maintaining the redox potential across the inner mitochondrial membrane in glycolysis. Since glycerol is a main subunit in lipid metabolism, its abundance can easily lead to an increase in triglyceride accumulation at a cellular level. As a result, there is a tendency to form adipose tissue leading to an accumulation of fat that favors obesity. GPDH has also been found to play a role in Brugada syndrome. Mutations in the gene encoding GPD1 have been proven to cause defects in the electron transport chain. This conflict with NAD+/NADH levels in the cell is believed to contribute to defects in cardiac sodium ion channel regulation and can lead to a lethal arrythmia during infancy.

Gene Name: GPD1

Gene ID: 100339469

UniProt: P08507