Alpha1 -Trypsin antibody (HRP)

Catalog No. ABIN5596883

Product Details

Target: Alpha1 -Trypsin

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Cross-Reactivity (Details): Cross reactivity against a1-Anti-Trypsin from other sources is unknown.

Purification: Anti-alpha-1-Anti-Trypsin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified a1-Anti-Trypsin [Human Plasma].

Immunogen:

Immunogen: Anti-alpha 1 Anti-Trypsin Antibody was produced by repeated immunizations with native alpha1-Anti-Trypsin protein from human plasma.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-α-1-anti-Trypsin Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight (~47 kDa) and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: Alpha1 -Trypsin

Alternative Name: Alpha-1-Trypsin

Background:

Synonyms: A1AT antibody, AAT antibody, Alpha 1 antiproteinase antibody, Alpha 1 antitrypsin antibody, Alpha 1 protease inhibitor antibod

Background: Anti-alpha 1 Anti-Trypsin Antibody detects a1-Anti-Trypsin protein. a1-Anti-Trypsin is an ihibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Anti-Trypsin irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form of Antitrypsin inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Defects in SERPINA1 are the cause emphysema, liver cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Anti-alpha 1 Anti-Trypsin Antibody is ideal for investigators involved in Cell Signaling, Signal Transduction and Cancer research.

Gene Name: SERPINA1

Gene ID: 5265

UniProt: P01009