ABCB8 antibody (Internal Region)

Catalog No. ABIN5596884

Product Details anti-ABCB8 Antibody

Target: ABCB8 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 8 (ABCB8))

Binding Specificity: Internal Region

Reactivity: Human, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABCB8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with ABCB8 from human, mouse, and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known. The antibody detects ABCB8 in cell lysates.

Purification: Anti-ABCB8 Antibody was prepared from monospecific, delipidated and defibrinated antiserum, with addition of sodium azide to 0.01%.

Immunogen:

Immunogen: ABCB8 was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a internal portion of human ABCB8.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-ABCB8 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect band approximately 79.9 kDa in size corresponding to ABCB8 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:250-1:500

ELISA Dilution: 1:20,000-1:50,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 80 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ABCB8

Target: ABCB8 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 8 (ABCB8))

Alternative Name: ABCB8 (ABCB8 Products)

Synonyms: EST328128 antibody, M-ABC1 antibody, MABC1 antibody, 4833412N02Rik antibody, AA409895 antibody, ABCB8 antibody, zgc:113037 antibody, ATP binding cassette subfamily B member 8 antibody, ATP-binding cassette, sub-family B (MDR/TAP), member 8 antibody, ABCB8 antibody, Abcb8 antibody, abcb8 antibody

Background:

Synonyms: ATP-binding cassette sub-family B member 8 mitochondrial, Mitochondrial ATP-binding cassette 1, M-ABC1, ABCB8

Background: This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer Research and drug resistance. ABCB8 is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). It has been found that ABC transporters are involved in mulitdrug resistance through their ability to contol the presence of drugs in cells. Multidrug resistance is currently being looked at in particular reference to cancer that is resistant to treatment. ABCB8 seems to have particulalrly high resistance rates in renal cancer and cancer of the central nervous system. Another significant aspect of ABCB8 is that it is a mitochondrial transporter. There are very few ABC transporters localized to the mitochondria which implies information about them could be extremely important. ABCB8 may also be localized to other parts of the cell which are yet to be determined. Anti-ABCB8 antibody is ideal for researchers nterested in Cancer Research and drug resistance.

Gene Name: ABCB8

Gene ID: 11194

UniProt: Q9NUT2