GFP antibody

Catalog No. ABIN559689

Cited in 1 publication. The Mouse Monoclonal anti-GFP antibody (Clone GF28R) (ABIN559689) specifically detects GFP in WB, ELISA, IP, DB and ISt. The antibody is reactive with Aequorea victoria samples.

Quick Overview for GFP antibody (ABIN559689)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB), Immunostaining (ISt)

Clone: GF28R

Product Details anti-GFP Antibody

Specificity: Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP

Sensitivity: WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins

Purification: Protein A affinity chromatography from mouse ascites fluid

Immunogen: GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates

Isotype: IgG2b

Application Details

Application Notes: WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Restrictions: For Research Use only

Handling

Concentration: 1mg/mL

Buffer: 10mM PBS, pH 7.2, 0.05% NaN3

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-GFP antibody (ABIN559689)

Chi, Bo, Wu, Jong, Huang: "Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells." in: PLoS ONE, Vol. 7, Issue 4, pp. e35862, (2012) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP