Trypsin antibody (HRP)

Catalog No. ABIN5596916

Product Details anti-Trypsin Antibody (HRP)

Target: Trypsin

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Trypsin antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity (Details): Cross reactivity against Trypsin from other sources is unknown.

Purification: Trypsin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsin [Bovine Pancreas].

Immunogen:

Immunogen: Anti-Trypsin Antibody was produced by repeated immunizations with bovine pancreatic trypsin protein purified.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Trypsin Antibody is suitable for western blotting and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:14,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Trypsin

Target: Trypsin

Abstract: Trypsin Products

Synonyms: wu:fb57g08 antibody, zgc:109701 antibody, LOC397853 antibody, Trypsin antibody, trp1 antibody, try1 antibody, trypsin antibody, protease, serine 1 antibody, protease, serine 1 L homeolog antibody, protease, serine, 1 antibody, trypsin antibody, prss1 antibody, prss1.L antibody, Deipr_2253 antibody

Background:

Synonyms: Anionic trypsinogen antibody, Beta trypsin antibody, Brain trypsinogen antibody, Cationic Trypsinogen antibody, Digestive Zymogen antibody, Mesotrypsin antibody, Mesotrypsinogen antibody

Background: Anti-Trypsin Antibody detects trypsin. Trypsin is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. It belongs to the peptidase S1 family. Trypsin is produced in the pancreas as the inactive proenzyme trypsinogen. It cleaves peptides on the C-terminal side of lysine and arginine amino acid residues. Trypsin hydrolyzes ester and amide linkages of several synthetic substrates. Anti-Trypsin Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction and Biochemistry research.

Gene Name: TRBOTR

Gene ID: 780933

UniProt: P00760