ERK1 antibody

Catalog No. ABIN559693

This Mouse Monoclonal antibody specifically detects ERK1 in WB and IP. It exhibits reactivity toward Human and Mouseand has been mentioned in 1 publication.

Quick Overview for ERK1 antibody (ABIN559693)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: E32

Product Details anti-ERK1 Antibody

Specificity: Recognizes only the ERK1 protein (e.g.: from human and mouse cells). WB: ERK1 from human and mouse cells / IP: ERK1 from human cells / ERK1 from other species may also be detectable

Purification: Protein A affinity chromatography from mouse ascites fluid

Immunogen: Recombinant full length ERK1 protein

Isotype: IgG1

Application Details

Application Notes: WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature) / For best results with other assays (e.g.: IP, etc), please determine optimal working dilution by titration test

Restrictions: For Research Use only

Handling

Concentration: 1mg/mL

Buffer: 10mM PBS, pH 7.2, 0.05% NaN3

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-ERK1 antibody (ABIN559693)

Johnson, Walker: "Cyclins and cell cycle checkpoints." in: Annual review of pharmacology and toxicology, Vol. 39, pp. 295-312, (1999) (PubMed).

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: ERK1

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins