Laminin antibody

Catalog No. ABIN5596935

Product Details anti-Laminin Antibody

Target: Laminin (LN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Laminin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity (Details): Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. Anti-Laminin (Human) (RABBIT) Antibody reacts with most mammalian Laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.

Purification: Anti-Laminin (RABBIT) Antibody has been prepared by immunoaffinity chromatography using immobilized human placental laminin followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities.

Immunogen:

Immunogen: Produced against a mixture of synthetic peptides corresponding to regions of adherence on laminin.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:50 - 1:200, 5 μg/mL

Application Note: Anti-Laminin (RABBIT) Antibody was assayed by immunoblot and found to be reactive against Laminin at a dilution of 1:5,000 to 1:10,000. Anti-Laminin (Human) (RABBIT) Antibody was also assayed against 1.0 μg of Laminin in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200. Specific conditions should be optimized by researcher.

Western Blot Dilution: 1:500 - 1:,000

ELISA Dilution: 1:4,000 - 1:8,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.04 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Laminin

Target: Laminin (LN)

Alternative Name: Laminin (LN Products)

Synonyms: LAMC1 antibody, laminin antibody, laminin, beta 2 (laminin S) antibody, LAMB2 antibody

Background:

Synonyms: LAMA1 antibody, LAMB1 antibody, Laminin A chain antibody, Laminin alpha 1 antibody, Laminin alpha 1 chain antibody, Laminin B1 chain antibody, Laminin subunit beta 1 antibody

Background: Laminin Antibody is specific to Laminin. Laminins are major proteins in the basal lamina, a protein network foundation for most cells and organs. The laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, adhesion as well as phenotype and survival. Anti-Laminin antibody is idea for investigators involved in cell cycle protein and extracelluar matrix research.

Gene Name: LAMB1

Gene ID: 3912

UniProt: P07942