Laminin antibody
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- Target See all Laminin (LN) Antibodies
- Laminin (LN)
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Laminin antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Cross-Reactivity (Details)
- Typically less than 1% cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian Laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross-reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.
- Purification
- Anti-Laminin has been prepared by immunoaffinity chromatography using immobilized human placental laminin followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities.
- Immunogen
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Immunogen: Anti-Laminin Antibody was produced by repeated immunizations with a mixture of synthetic peptides corresponding to regions of adherence on laminin.
Immunogen Type: Peptide
- Isotype
- IgG
- Top Product
- Discover our top product LN Primary Antibody
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- Application Notes
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Immunohistochemistry Dilution: 1:50 - 1:200
Application Note: Anti-Laminin antibody is suitable for western blotting, IHC, IP and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:500 - 1:2,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:4,000 - 1:8,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
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- Target
- Laminin (LN)
- Alternative Name
- Laminin (LN Products)
- Synonyms
- LAMC1 antibody, laminin antibody, laminin, beta 2 (laminin S) antibody, LAMB2 antibody
- Background
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Synonyms: LAMA1 antibody, LAMB1 antibody, Laminin A chain antibody, Laminin alpha 1 antibody, Laminin alpha 1 chain antibody, Laminin B1 chain antibody, Laminin subunit beta 1 antibody
Background: Anti-Laminin detects laminin. Laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, adhesion as well as phenotype and survival. The laminins are a family of glycoproteins that are an integral part of the structural scaffolding in almost every tissue of an organism. They are secreted and incorporated into cell-associated extracellular matrices. Defective laminins can cause muscles to form improperly, leading to a form of muscular dystrophy, lethal skin blistering disease and defects of the kidney filter. Anti-Laminin Antibody is ideal for investigators involved in Cell Signaling and Signal Transduction research.
Gene Name: LAMB1
- Gene ID
- 3912
- UniProt
- P07942
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