For best experience we recommend to activate Javascript in your browser.

Aurora A antibody (AA 268-404)

Name: Aurora A antibody (AA 268-404)
Brand: antibodies-online
SKU: ABIN5611121
Price: 533.5 USD
Availability: InStock

This Mouse Monoclonal antibody specifically detects Aurora A in WB, ELISA, IHC, ICC and FACS. It exhibits reactivity toward Human.

Western blot analysis using AURKA mAb against human AURKA (AA: 268-404) recombinant protein. (Aurora A antibody (AA 268-404))

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng) (Aurora A antibody (AA 268-404))

ELISA

Western blot analysis using AURKA mAb against HEK293 (1) and AURKA (AA: 268-404)-hIgGFc transfected HEK293 (2) cell lysate. (Aurora A antibody (AA 268-404))

Western blot analysis using AURKA mouse mAb against HEK293 (1), MCF-7 (2), and Hela (3) cell lysate. (Aurora A antibody (AA 268-404))

Flow cytometric analysis of HeLa cells using AURKA mouse mAb (green) and negative control (red). (Aurora A antibody (AA 268-404))

FACS

Immunofluorescence analysis of Hela cells using AURKA mouse mAb (green). (Aurora A antibody (AA 268-404))

Immunofluorescence analysis of SMMC-7721 cells using AURKA mouse mAb (green). (Aurora A antibody (AA 268-404))

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using AURKA mouse mAb with DAB staining. (Aurora A antibody (AA 268-404))

IHC

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using AURKA mouse mAb with DAB staining. (Aurora A antibody (AA 268-404))

IHC

9 images

Catalog No. ABIN5611121

Quick Overview for Aurora A antibody (AA 268-404) (ABIN5611121)

Target

See all Aurora A (AURKA) Antibodies

Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity

Human

Host

Mouse

Clonality

Monoclonal

Conjugate

This Aurora A antibody is un-conjugated

Application

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone

2D8A4

Binding Specificity
22

11

9

8

8

6

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 268-404

Purpose

AURKA Antibody

Purification

Purified antibody

Immunogen

Purified recombinant fragment of human AURKA (AA: 268-404) expressed in E. Coli.

Isotype

IgG2b
anti-Aurora Kinase A (AURKA) (C-Term) antibody
AURKA Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Aurora Kinase A (AURKA) (AA 1-110) antibody
AURKA Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 5F8 unconjugated

anti-Aurora Kinase A (AURKA) (AA 364-392), (C-Term) antibody
AURKA Reactivity: Human WB, IF, FACS Host: Rabbit Polyclonal RB6834 unconjugated

anti-Aurora Kinase A (AURKA) antibody
AURKA Reactivity: Human, Rat, Monkey WB, ELISA, ICC, FACS Host: Mouse Monoclonal 1F8 unconjugated

anti-Aurora Kinase A (AURKA) (pThr288) antibody
AURKA Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Aurora Kinase A (AURKA) (AA 268-404) antibody
AURKA Reactivity: Human, Mouse WB, ELISA, IHC Host: Mouse Monoclonal 4H5A8 unconjugated

anti-Aurora Kinase A (AURKA) antibody
AURKA Reactivity: Human, Rat, Monkey WB, ELISA, IHC Host: Mouse Monoclonal 4G10 unconjugated

anti-Aurora Kinase A (AURKA) (AA 364-392) antibody
AURKA Reactivity: Human WB, ELISA, IF, FACS Host: Rabbit Polyclonal unconjugated

anti-Aurora Kinase A (AURKA) (AA 1-131) antibody
AURKA Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Aurora Kinase A (AURKA) (AA 1-100) antibody
AURKA Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/200 - 1/1000

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Target

Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name

AURKA

Background

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.

Molecular Weight

45.8 kDa

UniProt

O14965

Pathways

Cell Division Cycle, Asymmetric Protein Localization