CKB antibody

Catalog No. ABIN5646868

The Mouse Monoclonal anti-CKB antibody has been validated for IF, FACS and IHC (fro). It is suitable to detect CKB in samples from Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Chicken, Frog and Rhesus Monkey.

Quick Overview for CKB antibody (ABIN5646868)

Target: CKB (Creatine Kinase, Brain (CKB))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Chicken, Frog, Rhesus Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CKB antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2ba6

Product Details anti-CKB Antibody

Purification: Purified

Purity: Protein G affinity chromatography

Immunogen: Human CKBB protein was used as the immunogen for the Creatine kinase B type antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the Creatine kinase B type antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (Frozen Only): 0.5-1 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Creatine kinase B type antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CKB

Target: CKB (Creatine Kinase, Brain (CKB))

Alternative Name: Creatine kinase B type / CKB

Background: Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This mAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteins