GCK antibody (AA 1-30)

Catalog No. ABIN5647784

Product Details anti-GCK Antibody

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Binding Specificity: AA 1-30

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCK antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Purified

Immunogen: A portion of amino acids 1-30 from the human protein was used as the immunogen for the GCK antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the GCK antibody should be determined by the researcher.\. Western Blot: 1:500-1:1000,IHC (FFPE): 1:50-1:100,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: After reconstitution, the GCK antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for GCK

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Alternative Name: GCK / Glucokinase (GCK Products)

Synonyms: FGQTL3 antibody, GK antibody, GLK antibody, HHF3 antibody, HK4 antibody, HKIV antibody, HXKP antibody, LGLK antibody, MODY2 antibody, Gk antibody, Gls006 antibody, GCK antibody, BA4487 antibody, glk antibody, hk4 antibody, hhf3 antibody, hkiv antibody, hxkp antibody, gck-A antibody, mody2 antibody, glucokinase antibody, GLUKA antibody, RNGK2 antibody, glucokinase antibody, glucokinase (hexokinase 4) L homeolog antibody, glucokinase (hexokinase 4) antibody, GCK antibody, Gck antibody, glcK antibody, Bphyt_1025 antibody, Halhy_6548 antibody, gk antibody, gck.L antibody, gck antibody

Background: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of the gene for GCK results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in the gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), also called maturity onset diabetes of the young, type 2 (MODY2), mutations have also been associated with persistent hyperinsulinemic hypoglycemia of infancy (PHHI).

UniProt: P35557

Pathways: MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process