PIK3CA antibody (AA 150-250) (Biotin)

Catalog No. ABIN5651073

The Rabbit Polyclonal anti-PIK3CA antibody has been validated for WB. It is suitable to detect PIK3CA in samples from Mouse.

Quick Overview for PIK3CA antibody (AA 150-250) (Biotin) (ABIN5651073)

Target: PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3CA antibody is conjugated to Biotin

Application: Western Blotting (WB)

Product Details anti-PIK3CA Antibody (Biotin)

Binding Specificity: AA 150-250

Specificity: Detects 55.7 kDa.

Purification: Peptide Affinity Purified

Immunogen: Synthetic peptide of Human PIK3CA (150-250 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5651073 was sufficient for detection of PIK3CA in 15 μg of mouse brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Target Details for PIK3CA

Target: PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

Alternative Name: PIK3CA

Gene ID: 5290

NCBI Accession: NP_006209

UniProt: P42336

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling