Recombinant alpha Tubulin antibody
Quick Overview for Recombinant alpha Tubulin antibody (ABIN5668283)
Target
See all alpha Tubulin (TUBA1) AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- Anti-Alpha-Tubulin [F2C], Rabbit IgG, lambda
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Specificity
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This antibody is specific for human alpha-tubulin (no cross-reactivity with beta-tubulin). Tubulin is the major constituent of microtubules. The alpha chain has a non-exchangeable GTP-binding site.
Application Notes original Clone: This antibody's original format (human scFv) was successfully used in a Western blot, and its anti-myc (9E10) complexed version was successfully used for in vitro immunofluorescence staining of alpha-tubulin in HeLa cells (Nizak et al., 2003, PMID: 14617357).
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Characteristics
- This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
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Purification
- Protein A affinity purified
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Immunogen
- The original antibody was generated via phage display from the Griffin.1 library by screening against Microtubule-Binding Proteins (MBPs) derived from HeLa S3 cells.
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Isotype
- IgG, lambda
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anti-alpha Tubulin (TUBA1) antibody
TUBA1 Reactivity: Human, Mouse, Pig, Saccharomyces cerevisiae, Arabidopsis, Nicotiana tabacum, Paramecium, Eisenia fetida, Turkey WB, IHC (p), ICC, IP, ICFC Host: Mouse Monoclonal TU-01 unconjugated
anti-alpha Tubulin (TUBA1) antibody (FITC)TUBA1 Reactivity: Human, Mouse, Pig, Saccharomyces cerevisiae, Arabidopsis, Nicotiana tabacum, Paramecium, Eisenia fetida, Turkey ICC, FACS Host: Mouse Monoclonal TU-01 FITC
anti-alpha Tubulin (TUBA1) antibody (Biotin)TUBA1 Reactivity: Human, Mouse, Pig, Saccharomyces cerevisiae, Arabidopsis, Nicotiana tabacum, Paramecium, Eisenia fetida, Turkey WB, IHC (p), ICC, ICFC Host: Mouse Monoclonal TU-01 Biotin
anti-alpha Tubulin (TUBA1) antibodyTUBA1 Reactivity: Human, Mouse, Rat, Pig, Nicotiana tabacum, Dog WB, ELISA, IHC (p), ICC, IP Host: Mouse Monoclonal TU-16 unconjugated
anti-alpha Tubulin (TUBA1) (AA 380-460) antibodyTUBA1 Reactivity: Human, Mouse, Rat, Chicken WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated
anti-alpha Tubulin (TUBA1) (acLys40) antibodyTUBA1 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated
anti-alpha Tubulin (TUBA1) (acLys40) antibodyTUBA1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-alpha Tubulin (TUBA1) antibodyTUBA1 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-alpha Tubulin (TUBA1) antibodyTUBA1 Reactivity: Human, Mouse, Rat, Pig, Cow, Saccharomyces cerevisiae, Monkey, Dog, Hamster, Guinea Pig, Amphibian, Sea Urchin, Goat, Kangaroo WB, IHC, IHC (p), IP Host: Mouse Monoclonal DM1A unconjugated
anti-alpha Tubulin (TUBA1) (Subunit alpha) antibodyTUBA1 Reactivity: Human, Saccharomyces cerevisiae, Mammalian, Avian, Drosophila melanogaster, Fungus WB, IHC, ELISA, IHC (p), IHC (fro), RIA Host: Rat Monoclonal YOL1-34 unconjugated
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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OriginalSpeciesName: Human
OriginalFormat: scFv
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Restrictions
- For Research Use only
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Concentration
- 1 mg/mL
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Buffer
- PBS with 0.02 % Proclin 300.
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Preservative
- ProClin
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Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- alpha Tubulin (TUBA1)
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Alternative Name
- Alpha-Tubulin
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Background
- TUBA, α-tubulin
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UniProt
- Q71U36
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Pathways
- Microtubule Dynamics
Target
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