Recombinant His Tag antibody (N-Term)

Catalog No. ABIN5671299

The Rabbit Chimeric anti-His Tag antibody is suitable to detect His Tag. It has been validated for WB and ELISA.

Quick Overview for Recombinant His Tag antibody (N-Term) (ABIN5671299)

Target: His Tag

Antibody Type: Recombinant Antibody

Host: Rabbit

Clonality: Chimeric

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: RMH01

Product Details anti-His Tag Antibody

Binding Specificity: N-Term

Purpose: Chimeric Human monoclonal to His-Tag. (6xHis-Tag or 10xHis-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy)

Specificity: This antibody is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (RM146), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.

Purification: Protein A affinity purified from an animal origin-free culture supernatant

Immunogen: Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Isotype: IgG

Application Details

Application Notes: ELISA: Capturer: 0. 1 μg/mL - 2 μg/mL, Detector: 0.01 μg/mL - 0.5 μg/mL, Western Blot: 0.1 μg/mL - 1 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 50 % Glycerol/PBS with 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20.0°C

Target Details for His Tag

Target: His Tag

Alternative Name: His-Tag

Target Type: Tag