Collagen IV antibody

Catalog No. ABIN5680248

This Rabbit Polyclonal antibody specifically detects Collagen IV in WB, ELISA, IHC (p), IF and RIA. It exhibits reactivity toward Mouse.

Quick Overview for Collagen IV antibody (ABIN5680248)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA)

Product Details anti-Collagen IV Antibody

Specificity: Murine collagen type IV 100 % , murine collagen type I, II and III <0.1 %, human collagen type IV and V <0.1 %, murine fibronectin <0.1 %, murine laminin <0.1 % in RIA at 1:5000 dilution

Cross-Reactivity (Details): Mouse

Characteristics: Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Murine collagen type IV 100 % , murine collagen type I, II and III <0.1 %, human collagen type IV and V <0.1 %, murine fibronectin <0.1 %, murine laminin <0.1 % in RIA at 1:5000 dilution.

Purification: Affinity purified

Immunogen: Purified collagen type IV from murine tumour tissue

Isotype: IgG

Application Details

Application Notes: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Mouse skin or liver,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 1 mg/mL

Buffer: phosphate buffered solution, no BSA and preservative added!

Preservative: Without preservative

Storage: -20 °C

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen Type IV