PFKM antibody (Biotin)

Catalog No. ABIN568195

This Sheep Polyclonal antibody specifically detects PFKM in WB, IF, IP, EIA, DB, ID and RIA. It exhibits reactivity toward Rabbit.

Quick Overview for PFKM antibody (Biotin) (ABIN568195)

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Reactivity: Rabbit

Host: Sheep

Clonality: Polyclonal

Conjugate: This PFKM antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)

Product Details anti-PFKM Antibody (Biotin)

Characteristics: Molar Ratio: Biotin/IgG ~6.5

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Fructose-6-Phosphate Kinase is isolated and purified from Rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for PFKM

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Alternative Name: PFKM

Background: Synonyms: 6-phosphofructokinase muscle type, GSD7, PFK-1, PFK-A, PFK1, PFKX, Phosphofructo-1-kinase isozyme A, Phosphofructokinase 1, Phosphofructokinase-M, Phosphohexokinase

Gene ID: 9986

UniProt: P00511

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect