PHGDH antibody

Catalog No. ABIN568368

The Sheep Polyclonal anti-PHGDH antibody (ABIN568368) specifically detects PHGDH in WB, IF, IP, EIA, RIA, ID and DB. The antibody is reactive with Saccharomyces cerevisiae samples.

Quick Overview for PHGDH antibody (ABIN568368)

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Reactivity: Saccharomyces cerevisiae

Host: Sheep

Clonality: Polyclonal

Conjugate: This PHGDH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Immunodiffusion (ID), Dot Blot (DB)

Product Details anti-PHGDH Antibody

Specificity: Affinity purified antibodies of polyclonal rabbit antiserum to 6-phosphogluconic dehydrogenase from yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Purification: Solid Phase Affinity Chromatography

Immunogen: 6-Phosphogluconic dehydrogenase isolated and purified from yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL sterile distilled water

Concentration: 2,0 mg/mL

Buffer: PBS, pH 7.2 stabilized with Dextran and without preservatives and foreign proteins

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -20 °C for longer.

Target Details for PHGDH

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Alternative Name: PGD / PGDH

Background: Synonyms: 6-phosphogluconate dehydrogenase decarboxylating

Gene ID: 856589

NCBI Accession: NP_012053

UniProt: P38720

Pathways: Metabolism of Steroid Hormones and Vitamin D, Warburg Effect