Fibrinogen antibody (HRP)
Quick Overview for Fibrinogen antibody (HRP) (ABIN568521)
Target
See all Fibrinogen AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Characteristics
- Molar Ratio: Peroxidase/IgG ~1.7
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Purification
- Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
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Immunogen
- Fibrinogen is isolated from fresh plasma after removing Prothrombin. Freund’s complete adjuvant is used in the first step of the immunization procedure
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Isotype
- IgG
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
- Restore by adding 1 mL of sterile distilled water.
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Concentration
- 10 mg/mL
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Buffer
- PBS, pH 7.2 without preservatives.
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Preservative
- Without preservative
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Handling Advice
- Avoid Repeated thawing and freezing.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.
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- Fibrinogen
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Background
- Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis, releasing the fibrinopeptides A and B (molecular weight 50,000-65,000) and forming fibrin monomers. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking under the influence of factor XIII. The predominant gamma chain of normal fibrinogen (MW 50,000, with higher variants) has a low affinity for platelet binding.Synonyms: FGA, FGB, FGG
Target
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