Choline Acetyltransferase antibody

Catalog No. ABIN5687549

This anti-Choline Acetyltransferase antibody is a Goat Polyclonal antibody detecting Choline Acetyltransferase in WB and IHC. Suitable for Human, Mouse, Rat, Chicken and Goat. This Primary Antibody has been cited in 1 publication.

Quick Overview for Choline Acetyltransferase antibody (ABIN5687549)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivity: Human, Mouse, Rat, Chicken, Goat

Host: Goat

Clonality: Polyclonal

Conjugate: This Choline Acetyltransferase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Choline Acetyltransferase Antibody

Specificity: Specific for the ~ 70k choline acetyltransferase protein

Purification: Affinity Purified

Immunogen: Native choline acetyltransferase purified from human placenta.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Choline Acetyltransferase antibody can be stored at -20°C and is stable at -20°C for at least 1 year.

References for anti-Choline Acetyltransferase antibody (ABIN5687549)

Gonzales, Pare, Wichmann, Smith: "GABAergic inputs from direct and indirect striatal projection neurons onto cholinergic interneurons in the primate putamen." in: The Journal of comparative neurology, Vol. 521, Issue 11, pp. 2502-22, (2014) (PubMed).

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: Choline Acetyltransferase

Background: Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).

Molecular Weight: 70 kDa

Gene ID: 1103

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development