Leptin antibody (Biotin)

Catalog No. ABIN5687650

The Goat Polyclonal anti-Leptin antibody has been validated for WB and ELISA. It is suitable to detect Leptin in samples from Rat.

Quick Overview for Leptin antibody (Biotin) (ABIN5687650)

Target: Leptin (LEP)

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Leptin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-Leptin Antibody (Biotin)

Purification: Anti-rat Leptin specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant rat Leptin (rat Leptin).

Application Details

Application Notes: ELISA:

To detect rat Leptin by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant rat Leptin.

To detect Rat Leptin by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat Leptin (XP-5213) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat Leptin.

Western Blot:

To detect rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: Leptin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: Leptin

Gene ID: 25608

UniProt: P50596

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process