This antibody is expected to recognize all isoforms (NP_001123952.1, NP_001123954.1, NP_001123955.1, NP_001129499.1). Reported variants represent identical protein: NP_001123953.1, NP_006287.2.
Purification
VRK2 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
VRK2 Antibody was raised against synthetic peptide PNSQKVDSQKAATK found at an internal region of VRK2.
ELISA: antibody detection limit dilution 1:4000. Western Blot: Approx 60 kDa band observed in nuclear lysates of cell line K562 (calculated MW of 58.1 kDa according to NP_001123954.1). Recommended concentration: 1-3μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
VRK2 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.