Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Purification
Bcl-rambo (monoclonal Rocky-2) antibody was purified from concentrated hybridoma tissue culture supernatant. Bcl-rambo (monoclonal Rocky-1) antibody was purified to ≥,95% in SDS-PAGE.
Immunogen
Bcl-rambo (monoclonal Rocky-2) antibody was raised against recombinant human Bcl-rambo.
BCL2L13
Reactivity: Human, Mouse, Rat
WB, ELISA, IHC, IF, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Bcl-rambo antibody can be used to recognize the C-terminus of human Bcl-rambo (aa. 224-450). It detects endogenous protein by Immunocytochemistry (1:300), Immunoprecipitation (1:100) and Western Blot (1:1,000). Method: Following fixation with 2 % paraformaldehyde/PBS, HELA cells were incubated with MAb to Bcl-rambo (human) (CT) (Rocky-2) at a 1:300 dilution. An anti-mouse IgG antibody conjugated with Alexa Fluor 488 was used for detection. This product is for research use only.
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS containing 0.02 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Bcl-rambo antibody can be stored at 4°C, stable for one year.