PLK1 antibody (pThr210)

Catalog No. ABIN5688913

The Rabbit Polyclonal anti-PLK1 antibody has been validated for WB. It is suitable to detect PLK1 in samples from Human, Mouse, Rat, Xenopus laevis, Cow, Dog and Zebrafish (Danio rerio).

Quick Overview for PLK1 antibody (pThr210) (ABIN5688913)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Human, Mouse, Rat, Xenopus laevis, Cow, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PLK1 Antibody

Binding Specificity: pThr210

Purification: Affinity Purified

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr210 of PLK1.

Application Details

Application Notes: The antibody has been directly tested for reactivity in Western blots in rat and human tissues. It is anticipated that the antibody will react with bovine, canine, mouse, non-human primate, Xenopus and Zebrafish tissues based on the fact that these species have 100 % homology with the amino acid sequence used as antigen.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: PLK1 antibody can be stored at -20°C and is stable at -20°C for at least 1 year.

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1

Background: Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004). Deregulated expression of PLK's is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005). It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser137 and Thr210 (van de Weerdt et al., 2005).

Molecular Weight: 66 kDa

Gene ID: 5347

UniProt: P53350

Pathways: Cell Division Cycle, M Phase