ATM antibody (AA 1979-1983)

Catalog No. ABIN5689237

The Rabbit Polyclonal anti-ATM antibody has been validated for WB and IF. It is suitable to detect ATM in samples from Human and Mouse.

Quick Overview for ATM antibody (AA 1979-1983) (ABIN5689237)

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATM antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-ATM Antibody

Binding Specificity: AA 1979-1983

Specificity: This antibody detects endogenous level of total ATM protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: ATM (Ab-1981) antibody was raised against a peptide sequence around aa.1979~1983 (E-G-S-Q-S) derived from Human ATM.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Alternative Name: ATM

Background: ATM encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates, thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 350 kDa

Gene ID: 472

NCBI Accession: NP_000042

UniProt: Q13315

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus