PAK1 antibody (AA 210-214)

Catalog No. ABIN5689498

The Rabbit Polyclonal anti-PAK1 antibody is suitable to detect PAK1 in samples from Human, Rat and Mouse. It has been validated for WB, IHC and IF.

Quick Overview for PAK1 antibody (AA 210-214) (ABIN5689498)

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PAK1 Antibody

Binding Specificity: AA 210-214

Specificity: This antibody detects endogenous level of total PAK1protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: PAK1 (Ab-212) antibody was raised against a peptide sequence around aa. 210~214 (P-V-T-P-T) derived from Human PAK1.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Alternative Name: PAK1

Background: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Molecular Weight: 68 kDa

Gene ID: 5058

NCBI Accession: NP_001122092

UniProt: Q13153

Pathways: MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis