FLT3 antibody (AA 589-593)

Catalog No. ABIN5689528

The Rabbit Polyclonal anti-FLT3 antibody has been validated for WB and IF. It is suitable to detect FLT3 in samples from Human.

Quick Overview for FLT3 antibody (AA 589-593) (ABIN5689528)

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-FLT3 Antibody

Binding Specificity: AA 589-593

Specificity: This antibody detects endogenous level of total FLT3 protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: FLT3 (Ab-591) antibody was raised against a peptide sequence around aa.589~593 (Y-F-Y-V-D) derived from Human FLT3.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for FLT3

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Alternative Name: FLT3

Background: FLT3 encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

Molecular Weight: 130, 160 kDa

Gene ID: 2322

NCBI Accession: NP_004110

UniProt: P36888

Pathways: RTK Signaling