Caspase 8 antibody (AA 221-225)

Catalog No. ABIN5689547

This anti-Caspase 8 antibody is a Rabbit Polyclonal antibody detecting Caspase 8 in WB. Suitable for Human.

Quick Overview for Caspase 8 antibody (AA 221-225) (ABIN5689547)

Target: Caspase 8 (CASP8)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 8 Antibody

Binding Specificity: AA 221-225

Specificity: This antibody detects endogenous level of total Caspase8 protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: Caspase8 antibody was raised against a peptide sequence around aa.221~225 (T-L-D-K-V) derived from Human Caspase8.

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: Caspase8

Background: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

Molecular Weight: 18, 43, 57 kDa

Gene ID: 58022

NCBI Accession: NP_571585

UniProt: Q14790

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades