ENOS antibody (AA 1175-1179)

Catalog No. ABIN5689588

The Rabbit Polyclonal anti-ENOS antibody is suitable to detect ENOS in samples from Human, Mouse and Rat. It has been validated for WB and IF.

Quick Overview for ENOS antibody (AA 1175-1179) (ABIN5689588)

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENOS antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-ENOS Antibody

Binding Specificity: AA 1175-1179

Specificity: This antibody detects endogenous level of total eNOS protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: eNOS (Ab-1177) antibody was raised against a peptide sequence around aa.1175~1179 (T-Q-S-F-S) derived from Human eNOS.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Alternative Name: eNOS

Background: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Molecular Weight: 140 kDa

Gene ID: 4846

NCBI Accession: NP_000594

UniProt: P29474

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling