Retinoblastoma 1 antibody (AA 805-809)

Catalog No. ABIN5689609

The Rabbit Polyclonal anti-Retinoblastoma 1 antibody has been validated for WB and IHC. It is suitable to detect Retinoblastoma 1 in samples from Human, Mouse and Rat.

Quick Overview for Retinoblastoma 1 antibody (AA 805-809) (ABIN5689609)

Target: Retinoblastoma 1 (RB1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoblastoma 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Retinoblastoma 1 Antibody

Binding Specificity: AA 805-809

Specificity: This antibody detects endogenous level of total Rb protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: Rb (Ab-807) antibody was raised against a peptide sequence around aa.805~809 (Y-I-S-P-L) derived from Human Rb.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: Rb

Background: Rb operates in the midst of the cell cycle clock apparatus. Its main role is to act as a signal transducer connecting the cell cycle clock with the transcriptional machinery (Ref.1). It plays an important role in the Rb/ E2F pathway in cell proliferation, cell fate determination, and cancer (Ref.2). Cellular senescence is a stable form of cell cycle arrest that limits proliferation of damaged cells and act as a natural barrier to cancer progression. A distinct heterochromatic structure that accumulates in senescent human fibroblasts, designated as SAHF (Senescence-Associated Heterochromatic Foci). SAHF formation coincides with recruitment of heterochromatin proteins and the Rb protein to E2F-responsive promoters and is associated with the stable repression of E2F target genes. Both SAHF formation and the silencing of E2F target genes depended on the integrity of the Rb pathway and do not occur in reversibly arrested cells (Ref.3). Rb activates transcription of the c-Jun gene through the SP1-binding site within the c-Jun promoter

Molecular Weight: 110 kDa

Gene ID: 5925

NCBI Accession: NP_000312

UniProt: P06400

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy