Tyrosine Hydroxylase antibody (AA 29-33)

Catalog No. ABIN5689619

The Rabbit Polyclonal anti-Tyrosine Hydroxylase antibody (ABIN5689619) specifically detects Tyrosine Hydroxylase in WB. The antibody is reactive with Rat and Mouse samples.

Quick Overview for Tyrosine Hydroxylase antibody (AA 29-33) (ABIN5689619)

Target: Tyrosine Hydroxylase (TH)

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Tyrosine Hydroxylase Antibody

Binding Specificity: AA 29-33

Specificity: This antibody detects endogenous levels of total Tyrosine Hydroxylase protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: Tyrosine Hydroxylase (Ab-31) antibody was raised against a peptide sequence around aa.29~33 (V-T-S-P-R) derived from Mouse Tyrosine Hydroxylase.

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Alternative Name: Tyrosine Hydroxylase

Background: Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms . This enzyme is required for embryonic development since TH knockout mice die before or at birth . Levels of transcription, translation and posttranslational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31 and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH . Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels.

Molecular Weight: 55 - 60 kDa

Gene ID: 7054

NCBI Accession: NP_954986

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour