CRH antibody (AA 30-80)

Catalog No. ABIN5689930

The Rabbit Polyclonal anti-CRH antibody is suitable to detect CRH in samples from Human. It has been validated for WB, ELISA, IF and IHC (p).

Quick Overview for CRH antibody (AA 30-80) (ABIN5689930)

Target: CRH (Corticotropin Releasing Hormone (CRH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CRH Antibody

Binding Specificity: AA 30-80

Specificity: CRH antibody is human specific.

Purification: CRH antibody is affinity chromatography purified via peptide column.

Immunogen: CRH antibody was raised against a 16 amino acid peptide near the amino terminus of human CRH.

The immunogen is located within amino acids 30 - 80 of CRH.

Isotype: IgG

Application Details

Application Notes: CRH antibody can be used for detection of CRH by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: CRH antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: CRH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for CRH

Target: CRH (Corticotropin Releasing Hormone (CRH))

Alternative Name: CRH

Background: Corticotropin-releasing hormone (CRH) is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress (1). In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta where it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturitio (2). Marked reduction in this protein has been observed in association with Alzheimer disease (3).

Molecular Weight: Predicted: 22 kDa

Observed: 29 kDa

Gene ID: 1392

NCBI Accession: NP_000747

UniProt: P06850

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour