CXCL14 antibody (Biotin)

Catalog No. ABIN5690515

The Rabbit Polyclonal anti-CXCL14 antibody is suitable to detect CXCL14 in samples from Human. It has been validated for ELISA and WB.

Quick Overview for CXCL14 antibody (Biotin) (ABIN5690515)

Target: CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL14 antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB)

Product Details anti-CXCL14 Antibody (Biotin)

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBRAK. Human BRAK specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect hBRAK by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBRAK.

Sandwich
To detect hBRAK by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human BRAK as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBRAK.

Western Blot:
To detect hBRAK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBRAK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: BRAK antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CXCL14

Target: CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

Alternative Name: BRAK

Gene ID: 9547

UniProt: O95715

Pathways: Autophagy