IGFBPI antibody (Biotin)

Catalog No. ABIN5690525

Product Details anti-IGFBPI Antibody (Biotin)

Target: IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGFBPI antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Human IGF-BP1. Human IGF-BP1 specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect hIGF-BP1 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP1.

Sandwich
To detect hIGF-BP1 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci's Polyclonal Anti-Human IGF-BP1 (38-265) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP1.

Western Blot:

To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IGFBPI

Target: IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

Alternative Name: IGF-BP1 (IGFBPI Products)

Synonyms: cb656 antibody, igfbp1 antibody, IGFBP-1 antibody, MGC68538 antibody, afbp antibody, ibp1 antibody, pp12 antibody, igf-bp25 antibody, higfbp-1 antibody, IGFBP1 antibody, LOC100223208 antibody, LOC100305121 antibody, AFBP antibody, IBP1 antibody, IGF-BP25 antibody, PP12 antibody, hIGFBP-1 antibody, IGFBA antibody, insulin like growth factor binding protein 1 antibody, insulin-like growth factor binding protein 1a antibody, insulin like growth factor binding protein 1 S homeolog antibody, insulin like growth factor binding protein 1 L homeolog antibody, insulin-like growth factor binding protein 1 antibody, IGFBP1 antibody, igfbp1a antibody, igfbp1.S antibody, igfbp1.L antibody, igfbp1 antibody, LOC100305121 antibody, Igfbp1 antibody

Gene ID: 3484

UniProt: P08833

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding