Tyrosine Hydroxylase antibody
Quick Overview for Tyrosine Hydroxylase antibody (ABIN5690708)
Target
See all Tyrosine Hydroxylase (TH) AntibodiesReactivity
Host
Clonality
Conjugate
Application
-
-
Purification
- Affinity Purified
-
Immunogen
- TH polyclonal antibody was raised against native rat tyrosine hydroxylase, purified from pheochromocytoma.
-
-
-
-
Application Notes
- Tyrosine Hydroxylase antibody is specific for the 60k rat TH in lysates of PC-12 Cells. Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blot (WB). The antibody has been characterized in blot immunolabeling and immunocytochemical studies and cross-reacts with many different mammalian and some non-mammalian forms of TH. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, IF and WB.
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Buffer
- 100 μLin 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.
-
Storage
- -20 °C
-
Storage Comment
- For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
-
-
- Tyrosine Hydroxylase (TH)
-
Alternative Name
- Tyrosine Hydroxylase
-
Background
- Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons.
-
Molecular Weight
- 60 kDa
-
Gene ID
- 25085
-
UniProt
- P04177
-
Pathways
- Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour
Target
-