The Sheep Polyclonal anti-Tyrosine Hydroxylase antibody (ABIN5690708) specifically detects Tyrosine Hydroxylase in WB, IHC and IF.
The antibody is reactive with Human, Rat, Mouse, Dog and Cow samples.
TH
Reactivity: Rat, Mouse
WB, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Tyrosine Hydroxylase antibody is specific for the 60k rat TH in lysates of PC-12 Cells. Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blot (WB). The antibody has been characterized in blot immunolabeling and immunocytochemical studies and cross-reacts with many different mammalian and some non-mammalian forms of TH. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, IF and WB.
Restrictions
For Research Use only
Format
Liquid
Buffer
100 μLin 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.
Storage
-20 °C
Storage Comment
For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Target
Tyrosine Hydroxylase (TH)
Alternative Name
Tyrosine Hydroxylase
Background
Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons.