Lamin A/C antibody (AA 319-566) (FITC)

Catalog No. ABIN5691105

This Mouse Monoclonal antibody specifically detects Lamin A/C in ICC, FACS and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Hamster and Sheep.

Quick Overview for Lamin A/C antibody (AA 319-566) (FITC) (ABIN5691105)

Target: Lamin A/C (LMNA)

Reactivity: Human, Mouse, Rat, Cow, Dog, Hamster, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin A/C antibody is conjugated to FITC

Application: Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 131C3

Product Details anti-Lamin A/C Antibody (FITC)

Binding Specificity: AA 319-566

Specificity: 131C3 reacts with an epitope located between residues 319-566 in lamin A and C.

Immunogen: 131C3 is a mouse monoclonal IgG1/k antibody derived by fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with purified rat liver lamins.

Isotype: IgG1

Application Details

Application Notes: 131C3 is suitable for immunocytochemistry, immunohistochemistry on frozen sections and flow cytometry. Optimal antibody dilutions for the different applications should be determined by titration. The recommended dilution is 1:10.

Restrictions: For Research Use only

Handling

Buffer: Each vial contains 1 mL FITC-conjugated anti lamin A and C monoclonal antibody in PBS containing 0.1 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C, or in small aliquots at -20°C.

Target Details for Lamin A/C

Target: Lamin A/C (LMNA)

Alternative Name: Lamin A and C

Background: Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.

Pathways: Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus