Lamin A/C antibody (AA 598-611) (FITC)

Catalog No. ABIN5691106

The Mouse Monoclonal anti-Lamin A/C antibody has been validated for ICC, FACS and IHC (fro). It is suitable to detect Lamin A/C in samples from Human, Mouse, Rat, Cow and Dog.

Quick Overview for Lamin A/C antibody (AA 598-611) (FITC) (ABIN5691106)

Target: Lamin A/C (LMNA)

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin A/C antibody is conjugated to FITC

Application: Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 133A2

Product Details anti-Lamin A/C Antibody (FITC)

Binding Specificity: AA 598-611

Specificity: 133A2 recognizes an epitope located between residues 598-611 of lamin A and therefore 133A2 reacts exclusively with lamin A.

Immunogen: 133A2 is a mouse monoclonal IgG3/kappa antibody obtained from fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with partially purified recombinant human lamin A.

Isotype: IgG3

Application Details

Application Notes: 133A2 is suitable for immunocytochemistry on permeabilized cells, immunohistochemistry on frozen sections and flow cytometry. Optimal antibody dilutions for the different applications should be determined by titration. The recommended dilution is 1:10.

Restrictions: For Research Use only

Handling

Buffer: Each vial contains 1 mL FITC-conjugated anti lamin A monoclonal antibody in PBS containing 0.1 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C, or in small aliquots at -20°C.

Target Details for Lamin A/C

Target: Lamin A/C (LMNA)

Alternative Name: Lamin A

Background: Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.

Pathways: Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus