Kallikrein 6 antibody (AA 22-244)

Catalog No. ABIN5692887

This Rabbit Polyclonal antibody specifically detects Kallikrein 6 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Kallikrein 6 antibody (AA 22-244) (ABIN5692887)

Target: Kallikrein 6 (KLK6)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Kallikrein 6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Kallikrein 6 Antibody

Binding Specificity: AA 22-244

Purpose: Anti-Kallikrein 6/KLK6 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Kallikrein 6/KLK6 Antibody Picoband® (ABIN5692887). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human Kallikrein 6 recombinant protein (Position: L22-K244).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Anisowicz, A., Sotiropoulou, G., Stenman, G., Mok, S. C., Sager, R. A novel protease homolog differentially expressed in breast and ovarian cancer. Molec. Med. 2: 624-636, 1996. 2. Elliott, M. B., Irwin, D. M., Diamandis, E. P. In silico identification and Bayesian phylogenetic analysis of multiple new mammalian kallikrein gene families. Genomics 88: 591-599, 2006. 3. Iwata, A., Maruyama, M., Akagi, T., Hashikawa, T., Kanazawa, I., Tsuji, S., Nukina, N. Alpha-synuclein degradation by serine protease neurosin: implication for pathogenesis of synucleinopathies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Kallikrein 6

Target: Kallikrein 6 (KLK6)

Alternative Name: KLK6

Background:

Synonyms: Kallikrein-6, Neurosin, Protease M, SP59, Serine protease 18, Serine protease 9, Zyme, KLK6, PRSS18, PRSS9

Tissue Specificity: In fluids, highest levels found in milk of lactating women followed by cerebrospinal fluid, nipple aspirate fluid and breast cyst fluid. Also found in serum, seminal plasma and some amniotic fluids and breast tumor cytosolic extracts. Not detected in urine. At the tissue level, highest concentrations found in glandular tissues such as salivary glands followed by lung, colon, fallopian tube, placenta, breast, pituitary and kidney. Not detected in skin, spleen, bone, thyroid, heart, ureter, liver, muscle, endometrium, testis, pancreas, seminal vesicle, ovary, adrenals and prostate. In brain, detected in gray matter neurons (at protein level). Colocalizes with pathological inclusions such as Lewy bodies and glial cytoplasmic inclusions. Overexpressed in primary breast tumors but not expressed in metastatic tumors.

Background: KLK6 (Kallikrein-related peptidase 6), also called KALLIKREIN 6, NEUROSIN, PROTEASE M, ZYME or PRSS9, is a protein that in humans is encoded by the KLK6 gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. Northern blot analysis revealed that the PRSS9 mRNA was expressed in several primary tumors and cell lines from mammary, prostate, and ovarian cancers, but was not detected in any metastases of these cancers. The KLK6 gene is mapped on 19q13.41. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease. Upon cellular stress, neurosin was released from mitochondria to the cytosol, which resulted in the increase of degraded alpha-synuclein species. Neurosin may play a significant role in physiologic alpha-synuclein degradation and also in the pathogenesis of synucleinopathies.

Molecular Weight: 29 kDa

Gene ID: 5653

UniProt: Q92876

Pathways: Complement System, Regulation of G-Protein Coupled Receptor Protein Signaling