Actin antibody

Catalog No. ABIN5692980

This Rabbit Monoclonal antibody specifically detects Actin in WB, IHC, IF, FACS, IP and ICC. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 5+ publications.

Quick Overview for Actin antibody (ABIN5692980)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C-1

Product Details anti-Actin Antibody

Purpose: Anti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal Antibody

Characteristics: Anti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal Antibody (ABIN5692980). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Actin (Alpha-Actin)

Isotype: IgG

Application Details

Application Notes: WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

References for anti-Actin antibody (ABIN5692980)

Cheng, Ji, Liu, Mei, Wang, Feng, Ding: "Erratum: Expression changes of ionic channels in early phase of cultured rat atrial myocytes induced by rapid pacing." in: Journal of cardiothoracic surgery, Vol. 10, pp. 10, (2015) (PubMed).

Cheng, Zhao, Wang, Lu, Wang, Wang, Yao: "The effect of 5'-adenylic acid on hepatic proteome of mice radiated by 60Co γ-ray." in: International journal of molecular sciences, Vol. 15, Issue 1, pp. 186-202, (2014) (PubMed).

Li, Chen, Li, Zhang, Tang: "Cytotoxicity of polyaniline nanomaterial on rat celiac macrophages in vitro." in: PLoS ONE, Vol. 9, Issue 9, pp. e107361, (2014) (PubMed).

Xing, Lv, Wang, Yan, Zhao, Cao: "Engineered myocardial tissues constructed in vivo using cardiomyocyte-like cells derived from bone marrow mesenchymal stem cells in rats." in: Journal of biomedical science, Vol. 19, pp. 6, (2012) (PubMed).

Ni, Chen, Yao, Yang, Liu, Wu: "Inhibition of human La protein by RNA interference downregulates hepatitis B virus mRNA in 2.2.15 cells." in: World journal of gastroenterology, Vol. 10, Issue 14, pp. 2050-4, (2004) (PubMed).

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: ACTA1

Background:

Synonyms: Actin, alpha skeletal muscle,Alpha-actin-1,ACTA1,ACTA,

Tissue Specificity: Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. .

Molecular Weight: 66 kDa

UniProt: P68133

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development