MAP2 antibody (AA 64-133)

Catalog No. ABIN5693007

Product Details anti-MAP2 Antibody

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Binding Specificity: AA 64-133

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Brand: Picoband™

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for MAP2 detection. Tested with WB, Direct ELISA in Human,Mouse,Rat.

Immunogen: E. coli-derived human MAP2 recombinant protein (Position: Q64-L133).

Application Details

Application Notes:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Application Details: Western blot, 0.1-0.5 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

References for anti-MAP2 antibody (ABIN5693007)

Wang, Xu, Chen, Xu, Qiu, Ge, Yu, Zhuang: "Brca1 Is Upregulated by 5-Aza-CdR and Promotes DNA Repair and Cell Survival, and Inhibits Neurite Outgrowth in Rat Retinal Neurons." in: International journal of molecular sciences, Vol. 19, Issue 4, (2018) (PubMed).

Li, Zhang, Liu, Han, Xu, Ma: "Neuroprotective effects of leonurine against oxygen-glucose deprivation by targeting Cx36/CaMKII in PC12 cells." in: PLoS ONE, Vol. 13, Issue 7, pp. e0200705, (2018) (PubMed).

Xiao, Liu, Zhang, Zou, Deng, Sun, Bian, Cai, Guo, Liu, Li, Shiang, Zhong, Cheng, Xu, Jin, Xiang: "Direct reprogramming of fibroblasts into neural stem cells by single non-neural progenitor transcription factor Ptf1a." in: Nature communications, Vol. 9, Issue 1, pp. 2865, (2018) (PubMed).

Yang, Wu, Tian, Li, Hu, Chen, Cai, Xu, Zhang, Chen, Ge, Yu, Zhuang: "Lithium promotes DNA stability and survival of ischemic retinal neurocytes by upregulating DNA ligase IV." in: Cell death & disease, Vol. 7, Issue 11, pp. e2473, (2017) (PubMed).

Liu, Lai, Zhao, Chen: "Increased expression of Myc-interacting zinc finger protein 1 in APP/PS1 mice." in: Experimental and therapeutic medicine, Vol. 14, Issue 6, pp. 5751-5756, (2017) (PubMed).

Han, Ge, Tan, Chen, Gao, Lei, Zhang: "Establishment of Lipofection Protocol for Efficient miR-21 Transfection into Cortical Neurons In Vitro." in: DNA and cell biology, Vol. 34, Issue 12, pp. 703-9, (2016) (PubMed).

Lin, Zheng, Zhang, Chen, Liao, Wan: "Effects of Tetramethylpyrazine on Functional Recovery and Neuronal Dendritic Plasticity after Experimental Stroke." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 394926, (2015) (PubMed).

Fan, Tian, Wang, Xu, Ren, Zhang, Gao, Shi, Meng, Zhang, Zhao, Shao, Dong: "Activation of the AMPK-ULK1 pathway plays an important role in autophagy during prion infection." in: Scientific reports, Vol. 5, pp. 14728, (2015) (PubMed).

Yan, Wang, Jiang, Chen, He, Mang, Shao, Xu: "The role of Rho/Rho-kinase pathway and the neuroprotective effects of fasudil in chronic cerebral ischemia." in: Neural regeneration research, Vol. 10, Issue 9, pp. 1441-9, (2015) (PubMed).

Chen, Hu, Chen, Cai, Zhang, Yang, Yu, Zhang, Xia, Ge, Yu, Zhuang: "BRCA1 silencing is associated with failure of DNA repairing in retinal neurocytes." in: PLoS ONE, Vol. 9, Issue 6, pp. e99371, (2014) (PubMed).

Fu, Lv, Zhuang, Chen, Lv, Li, Wang: "An effective inducer of dopaminergic neuron-like differentiation." in: Neural regeneration research, Vol. 8, Issue 5, pp. 427-34, (2014) (PubMed).

Wang, Ren, Guo, Yang, Long, Hu, Shen, Wang, Zeng: "The inhibitory effects of Npas4 on seizures in pilocarpine-induced epileptic rats." in: PLoS ONE, Vol. 9, Issue 12, pp. e115801, (2014) (PubMed).

Chen, Feng, Lin, Zhang, Li, Liang, Li: "Penicillamine increases free copper and enhances oxidative stress in the brain of toxic milk mice." in: PLoS ONE, Vol. 7, Issue 5, pp. e37709, (2012) (PubMed).

Sun, Jiang, Zhang, Chen, Xiang, Qi, Gao, Huang, Ge: "Gene expression and differentiation characteristics in mice E13.5 and E17.5 neural retinal progenitors." in: Molecular vision, Vol. 15, pp. 2503-14, (2009) (PubMed).

Target Details for MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Alternative Name: MAP2 (MAP2 Products)

Synonyms: MAP2A antibody, MAP2B antibody, MAP2C antibody, MAP2R antibody, Mtap2 antibody, MAP-2 antibody, Mtap-2 antibody, xmap2 antibody, MGC53131 antibody, a730034c02 antibody, zgc:103474 antibody, zgc:113974 antibody, microtubule associated protein 2 antibody, microtubule-associated protein 2 antibody, microtubule associated protein 2 S homeolog antibody, MAP2 antibody, Map2 antibody, map2.S antibody, map2 antibody

Background:

Synonyms: Microtubule-associated protein 2, MAP-2, MAP2

Background: Microtubule-associated protein 2 is a protein that in humans is encoded by the MAP2 gene. This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described.

UniProt: P11137