GRK2 antibody

Catalog No. ABIN5693069

This anti-GRK2 antibody is a Rabbit Polyclonal antibody detecting GRK2 in WB, IHC, ICC, IF and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for GRK2 antibody (ABIN5693069)

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-GRK2 Antibody

Purpose: Anti-GRK2/GRK2 Antibody Picoband®

Sequence: DSDPELVQWK KELRDAYREA QQLVQRVPKM KNK

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human

Characteristics: Anti-GRK2/GRK2 Antibody Picoband® (ABIN5693069). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GRK2, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Benovic JL, DeBlasi A, Stone WC, Caron MG, Lefkowitz RJ (1989). "Beta-adrenergic receptor kinase: primary structure delineates a multigene family". Science. 246 (4927): 235-40. 2. Pippig S, Andexinger S, Daniel K, Puzicha M, Caron MG, Lefkowitz RJ, Lohse MJ (1993). "Overexpression of beta-arrestin and beta-adrenergic receptor kinase augment desensitization of beta 2-adrenergic receptors".J. Biol. Chem. 268 (5): 3201-8.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GRK2

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Alternative Name: GRK2

Background:

Synonyms: Beta-adrenergic receptor kinase 1, Beta-ARK-1, G-protein coupled receptor kinase 2

Tissue Specificity: Expressed in peripheral blood leukocytes.

Background: Short transient receptor potential channel 3 (TrpC3) also known as transient receptor protein 3 (TRP-3) is a protein that in humans is encoded by the TRPC3 gene. The protein encoded by this gene is a membrane protein that can form a non-selective channel permeable to calcium and other cations. The encoded protein appears to be induced to form channels by a receptor tyrosine kinase-activated phosphatidylinositol second messenger system and also by depletion of intracellular calcium stores. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 79 kDa

Gene ID: 2156

UniProt: P25098

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling